Type II Collagen Detection ELISA Kit

Catalog # 6018

DESCRIPTION: ELISA kit to quantify multi-species Type II collagen

FORMAT: 96-well ELISA plate with removeable strips

ASSAY TYPE: Sandwich ELISA

ASSAY TIME: 3.5 hours (1-step protocol) 5.5 hours (2-step protocol)

STANDARD RANGE: 200 ng/ml - 3.1 ng/ml

NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate

SAMPLE TYPES: Solubilized collagen and cell culture media

RECOMMENDED SAMPLE DILUTIONS: 1:1 - 1:1000

CHROMOGEN: OPD (read at 490 nm)

STORAGE: -20°C for 12 months

VALIDATION DATA: Intra-assay (2.1-4.4%)/Inter-assay (6.3-9.3%)/Spiking Test (86-97%)

NOTES: This kit has the option to run the standard and detection antibody steps in either a 1-step or 2- step procedure